Journal: EMBO reports
Article Title: miRNA-mediated control of gephyrin synthesis drives sustained inhibitory synaptic plasticity.
doi: 10.1038/s44319-024-00253-z
Figure Lengend Snippet: Figure 2. miR153 overexpression disrupts gephyrin and GABAAR synaptic clustering.
Article Snippet: Membranes were incubated at 4 °C overnight with the appropriate primary antibody: GPHN (1:5000 Synaptic Systems 147111), GABAARα1 (1:1000 NeuroMab 75136), GABAARγ2 (1:1000 NeuroMab 75442), GAPDH (1:10,000 GeneTex 627408), GFP (1:2000 NeuroMab 75131), GABAAR β3 (1:1000 NeuroMab 75149), GABAARα5 (1:1000 NeuroMab 455510), GluA1 (1:1000 Millipore ABN241), NL2 (1:2500 Synaptic Systems 129511), CB (1:1000 Synaptic Systems 261 003), VAMP2 (1:5000 Synaptic Systems 104211).
Techniques: Over Expression